Opening

Full Time 40 hours Grade 079 Med M&D-Aab CardiovascResInst

Schedule

8 AM-4:30 PM

Responsibilities

GENERAL PURPOSE:

The Yao lab in the Cardiovascular Research Institute is looking for a highly motivated Laboratory Technician to assist with animal and cell-based experiments and with maintaining a mouse colony in the general support of a molecular biology research laboratory. The individual will be expected to achieve the ability to function independently. Excellent organizational skills and the ability to multi-task are required. All necessary training will be provided, however experience in mouse handling, mammalian cell culture and transfection, and molecular biology techniques, such as Western blotting, PCR, qPCR, cloning, etc, are a plus. Under general guidance from the PI with latitude for exercising considerable independent judgment of handling lab techniques. This position has no official supervisory responsibility.

RESPONSIBILITIES:

• Perform molecular biology techniques and experiments in support of a research lab, ex. bacterial culture, mini- and maxi-prep of plasmid DNA, maintainence of mammalian cell culture lines, protein purification, etc.
• Animal colony management for mouse colonies. Animal husbandry to include breeding, weaning, pairing, tail snip, and inventory maintenance. Data entry into established database and spreadsheet for management of animal colonies; inventory control of animal numbers and UCAR accounts
• Perform tissue harvests from adult mice and staged embryos and process using experimental procedures related to gene expression analysis (realtime PCR, western blot, immunostaining, WGA and picrosirius staining, etc.).
• Independently and proactively perform general laboratory duties such as preparing various culture media (mammalian and bacterial), chemical solutions, reagents and re-stocking supplies for use by all lab members. Inventory lab materials and order supplies, and tracking spending.
• Write new laboratory formulae, procedures and protocols. Perform the technical training for new lab members including postdoctoral fellows in the lab: including animal handing and breeding, mouse genotyping, Western blotting, bacteria plasmid culture and DNA purification.

Other duties as assigned

QUALIFICATIONS:

• Associate's degree in appropriate discipline (preferably Biology) required
• Or related field plus 3-4 years specialized experience in related field; or equivalent combination of education and experience.
• Previous work experience in a research lab highly preferred.
• Mac, PC, and MS Office. Pipettes, gel apparatus, incubator shaker, centrifuges, -80 freezer, Barnstead Water Units, NuAire Bio-safety cabinet, dissecting microscopes, various surgical instruments, PCR machine, down draft tables, perfusion apparatus, CO2 euthanasia stations, ventilated rodent housing racks preferred

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How To Apply

All applicants must apply online.

EOE Minorities/Females/Protected Veterans/Disabled

Pay Range

Pay Range: $20.92 - $29.92 Hourly

The referenced pay range represents the minimum and maximum compensation for this job. Individual annual salaries/hourly rates will be set within the job's compensation range, and will be determined by considering factors including, but not limited to, market data, education, experience, qualifications, expertise of the individual, and internal equity considerations.