Research Assistant III/IV - Human Genetics - Baglaenko Lab

Expected Starting Salary Range: 23.02 - 28.06 (RAIII) 26.42-32.71 (RAIV)

The self-motivated applicant will work in the Baglaenko Lab within the Division of Genetics. This human immunology focused group studies the basis of autoimmune disease using techniques in genomics, genetics, and systems biology with an emphasis on technology development. This position will focus on understanding the genetic drivers of human B cell responses using novel sequencing approaches.

Working under the general supervision of the Principal Investigator and in accordance with established hospital policies and procedures, the qualified applicant will conduct basic immunological and molecular biology techniques including sterile cell culture, MACS sorting of human PBMCs, and RNA/DNA extraction. Experience in Flow Cytometry, PCR, CRISPR, cloning, liquid handling automation, and maintenance of cell lines is not required but desirable. Data will be analyzed using R and Flowjo. The ideal candidate will have experience in sterile cell culture techniques, basic cellular immunology techniques and concepts, and a strong interest in developing a career in medical sciences. The position offers a unique opportunity to expand research methodologies within the rich scientific community of Cincinnati Children's Hospital.

REPRESENTATIVE RESPONSIBILITIES

• Perform experiments and record data

Follow established research protocols. Understand the scientific principles involved in the research. Apply those principles to troubleshoot and modify procedures and techniques when appropriate. Maintain accurate and detailed records of all laboratory work and experiments. Analyze data and perform statistical and graphical analysis of data. Design and execute daily laboratory experiments in coordination with senior laboratory personnel. Apply standard protocols to specific experiments. Assists in training post doctoral fellows, graduate students, and junior laboratory personnel in laboratory techniques and equipment. Capable of directing an undergraduate trainee in simple, well-established procedures. Maintains an active role in laboratory's meetings, presentations, and publications. Contributes material for reports, publications, journal articles, and abstracts. Able to orally present data in group presentations.

• Research and improve current methods

Utilize internal and external resources to reproduce, evaluate, research, and improve techniques, bring in new techniques or modify existing techniques. Evaluate innovative technique

• Lab Administration

Troubleshoot and correct erroneous results or problems with laboratory equipment. Troubleshoot and/or make modifications as necessary to established procedures. Maintain inventory of laboratory supplies and equipment. Maintain cell cultures. Operate standard laboratory equipment. Willing and able to assist in daily operations of the laboratory including answering phone, maintaining lab supplies, organization, and cleanliness. Maintains optimal protocol and assay accuracy by conducting regular quality checks. Provides appropriate safety monitoring checks as assigned (radiation safety, biosafety, etc.)

• Serve as a Resource

Demonstrate competence in specific knowledge, skills and behaviors. Effectively communicate with team members and contribute to their growth by sharing knowledge, experience, and skills. Stay abreast of changes in key systems. Serve as a resource to the department in specific area(s).

• Customer Service

Maintain/manage strong working relationships with clients, colleagues, and other key stakeholders. Respond to requests quickly and effectively. Identify and understand customer needs and expectations and meets them. Communicate any relevant information to clients, colleagues, and other key stakeholders.

Qualifications:

EDUCATION/EXPERIENCE

Required for RAIII:

• Bachelor's degree in a related field AND 1 year of directly related experience OR Master's degree in a related field

Required for RAIV:

• Bachelor's degree in a related field

• 3 years of experience in related job discipline OR Master's degree and 2 years experience

Cincinnati Children's is proud to be an Equal Opportunity Employer that values and treasures Diversity, Equity, and Inclusion. We are committed to creating an environment of dignity and respect for all our employees, patients, and families. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, age, genetic information, national origin, sexual orientation, gender identity, disability or protected veteran status. EEO/AA/M/F/Veteran/Disability