Research Technician I
Somerville, Massachusetts
Employer: Massachusetts General Hospital(MGH)
Industry: Technician/Technologist/Scientist
Salary: Competitive
Job type: Full-Time
GENERAL SUMMARY/ OVERVIEW STATEMENT:
The incumbent of this position will serve as a Research Technician I in the Collaborative Center for X-Linked Dystonia-Parkinsonism (CCXDP) within the Department of Neurology at Massachusetts General Hospital (MGH). This individual will participate in a broad range of experiments characterizing disease mechanisms underlying XDP, which is a rare neurodegenerative disease endemic to the Philippines. These studies will involve analyses of human patient-specific induced pluripotent stem cell (iPSC) models and postmortem tissue collected from individuals with XDP.
For cell modeling experiments, this individual will be responsible for propagating human iPSCs in culture and differentiating these lines into neuronal and glial lineages. These iPSC derivatives will be analyzed in multiple assays using combinations of molecular and biochemical readouts to identify cellular phenotypes associated with XDP.
For work with human XDP postmortem brain biospecimens, this individual will be responsible for preparing frozen tissue samples by cryostat sectioning, maintaining a detailed inventory of available biospecimens for distribution, and performing histologic and molecular analyses on processed samples.
The incumbent of this position will be expected to work independently with minimal supervision, participate directly in developing new methods and designing experiments, and carry out experiments and data analyses with precision.
PRINCIPAL DUTIES AND RESPONSIBILITIES:
Major job duties will involve:
Qualifications
SKILLS/ABILITIES/COMPETENCIES REQUIRED:
Must be able to work well as part of a team effort.
Excellent attention to detail and organizational skills.
Demonstrated knowledge of standard software programs (i.e. Excel, GraphPad, Photoshop, Powerpoint, etc.).
Strong verbal and written communication skills.
Ability to effectively multi-task.
EDUCATION:
BA/BS in relevant biomedical discipline required; previous coursework in neuroscience and/or stem cell biology is a plus but not required.
EXPERIENCE:
Strong preference given to candidates with experience working with iPSC lines.
Basic molecular biological techniques (RNA and DNA isolation and analysis, PCR amplification, DNA transfection), and basic protein biochemical techniques (SDS-PAGE and western blotting, immunofluorescence) are also preferred.
WORKING CONDITIONS:
All work performed in the MGH-East research campus in Charlestown, within a highly dynamic research group consisting of basic and clinical faculty members, postdoctoral fellows, technicians, and laboratory rotation students.
EEO Statement
Massachusetts General Hospital is an Affirmative Action Employer. By embracing diverse skills, perspectives and ideas, we choose to lead. All qualified applicants will receive consideration for employment without regard to race, color, religious creed, national origin, sex, age, gender identity, disability, sexual orientation, military service, genetic information, and/or other status protected under law. We will ensure that all individuals with a disability are provided a reasonable accommodation to participate in the job application or interview process, to perform essential job functions, and to receive other benefits and privileges of employment.
The incumbent of this position will serve as a Research Technician I in the Collaborative Center for X-Linked Dystonia-Parkinsonism (CCXDP) within the Department of Neurology at Massachusetts General Hospital (MGH). This individual will participate in a broad range of experiments characterizing disease mechanisms underlying XDP, which is a rare neurodegenerative disease endemic to the Philippines. These studies will involve analyses of human patient-specific induced pluripotent stem cell (iPSC) models and postmortem tissue collected from individuals with XDP.
For cell modeling experiments, this individual will be responsible for propagating human iPSCs in culture and differentiating these lines into neuronal and glial lineages. These iPSC derivatives will be analyzed in multiple assays using combinations of molecular and biochemical readouts to identify cellular phenotypes associated with XDP.
For work with human XDP postmortem brain biospecimens, this individual will be responsible for preparing frozen tissue samples by cryostat sectioning, maintaining a detailed inventory of available biospecimens for distribution, and performing histologic and molecular analyses on processed samples.
The incumbent of this position will be expected to work independently with minimal supervision, participate directly in developing new methods and designing experiments, and carry out experiments and data analyses with precision.
PRINCIPAL DUTIES AND RESPONSIBILITIES:
Major job duties will involve:
- Maintains a detailed inventory of barcoded human post-mortem frozen tissue blocks and processed biospecimens in the center's biobank, with detailed tracking information related to receipt and distribution details.
- Processes frozen human tissue blocks to generate mounted slides and tissue aliquots for use in histologic, molecular, and biochemical experiments.
- Performs histologic and molecular assays on tissue biospecimens, while also contributing to ongoing experiments involving cell lines to complement studies of brain tissue.
- Carries out required biosafety procedures and maintains approved biosafety protocols as required for work with human post-mortem tissue.
- Propagates and differentiates human patient and control iPSC lines.
- Analyzes cellular models using assays based on high-content fluorescence imaging, western blotting, ELISA, qRT-PCR, and related biochemical and molecular techniques.
Qualifications
SKILLS/ABILITIES/COMPETENCIES REQUIRED:
Must be able to work well as part of a team effort.
Excellent attention to detail and organizational skills.
Demonstrated knowledge of standard software programs (i.e. Excel, GraphPad, Photoshop, Powerpoint, etc.).
Strong verbal and written communication skills.
Ability to effectively multi-task.
EDUCATION:
BA/BS in relevant biomedical discipline required; previous coursework in neuroscience and/or stem cell biology is a plus but not required.
EXPERIENCE:
Strong preference given to candidates with experience working with iPSC lines.
Basic molecular biological techniques (RNA and DNA isolation and analysis, PCR amplification, DNA transfection), and basic protein biochemical techniques (SDS-PAGE and western blotting, immunofluorescence) are also preferred.
WORKING CONDITIONS:
All work performed in the MGH-East research campus in Charlestown, within a highly dynamic research group consisting of basic and clinical faculty members, postdoctoral fellows, technicians, and laboratory rotation students.
EEO Statement
Massachusetts General Hospital is an Affirmative Action Employer. By embracing diverse skills, perspectives and ideas, we choose to lead. All qualified applicants will receive consideration for employment without regard to race, color, religious creed, national origin, sex, age, gender identity, disability, sexual orientation, military service, genetic information, and/or other status protected under law. We will ensure that all individuals with a disability are provided a reasonable accommodation to participate in the job application or interview process, to perform essential job functions, and to receive other benefits and privileges of employment.
Created: 2024-05-08
Reference: 3289047
Country: United States
State: Massachusetts
City: Somerville
Similar jobs:
-
Clinical Care Technician, Surgical Day Care, 40 Hours, Days/Evenings
heywood in Gardner, Massachusetts -
Paramedic Health Technician
Massachusetts General Hospital(MGH) in Boston, Massachusetts -
Safety Technician
U.S. Army Reserve Command in Westover Air Reserve Base, Massachusetts -
Quality Control Technician
ASK Staffing in Devens, Massachusetts💸 $30 - $32.22 per hour -
Research Technician, Cancer Center
Massachusetts General Hospital(MGH) in Charlestown, Massachusetts -
Network Technician
Mass General Brigham in Boston, Massachusetts -
HVAC Technician III - Energy Systems - Massachusetts
Energy Systems East in Marlborough, Massachusetts -
Research Technician II
Massachusetts General Hospital(MGH) in Boston, Massachusetts -
Lead Appliance Repair Technician
Mr. Appliance in Woburn, Massachusetts💸 $31.00 - $36.00 per hour -
Facilities Maintenance Technician - Boston, MA
AutoZone in East Boston, Massachusetts -
Technician
FedEx in North Billerica, Massachusetts -
Surgical Technician -Operating Department\u003B 40 hours/week!$10K Sign On Bonus
Emerson Hospital in Concord, Massachusetts -
Clinical Investigation Technician I BWH Sleep Medicine
Brigham & Women's Hospital(BWH) in Boston, Massachusetts -
Pharmacy Technician
Big Y Foods, Inc in Wilbraham, Massachusetts -
Mechanic / Technician
Transforce in North Billerica, Massachusetts💸 $32.24 per hour -
Mobile Heavy Duty Service Technician w/CDL
Kenworth Northeast, Group Inc in Brockton, Massachusetts💸 $23 - $50 per hour -
Auto Mechanic / Technician II - ADESA Boston
Carvana in Framingham, Massachusetts -
Research Technician - Bioengineering
Tufts University in Medford, Massachusetts💸 $19.80 per hour -
PT Pharmacy Technician Certified - Pharmacy - 0011
Stop & Shop in Revere, Massachusetts -
PHARMACY TECHNICIAN I
South Shore Health in Weymouth, Massachusetts