Laboratory Technician III
Rochester, New York
Employer: University of Rochester
Industry: Healthcare Professional
Salary: $21.36 - $29.90 per hour
Job type: Full-Time
Opening
Full Time 40 hours URG Range 106 Pediatrics M&D Neonatology
Schedule
8:30 AM - 4:30 PM; OCC WKENDS
Responsibilities
General Purpose:
This position requires active participation in research laboratory activities. This individual will participate in basic science laboratory procedures including routine experiments using both cell culture and transgenic mouse models of erythropoiesis. Research will require experience with cell culture, mouse breeding/genotyping, FACS analyses, polymerase chain reaction, cloning techniques, tissue culture procedures, and fluorescent antibody detection. This position will involve the generation and maintenance of cell lines, handling mice, and analyzing erythropoiesis in cell culture and animal models.
Responsibilities:
Cell culture
Qualifications:
How To Apply
All applicants must apply online.
EOE Minorities/Females/Protected Veterans/Disabled
Pay Range
Pay Range: $21.36 - $29.90 Hourly
The referenced pay range represents the minimum and maximum compensation for this job. Individual annual salaries/hourly rates will be set within the job's compensation range, and will be determined by considering factors including, but not limited to, market data, education, experience, qualifications, expertise of the individual, and internal equity considerations.
Full Time 40 hours URG Range 106 Pediatrics M&D Neonatology
Schedule
8:30 AM - 4:30 PM; OCC WKENDS
Responsibilities
General Purpose:
This position requires active participation in research laboratory activities. This individual will participate in basic science laboratory procedures including routine experiments using both cell culture and transgenic mouse models of erythropoiesis. Research will require experience with cell culture, mouse breeding/genotyping, FACS analyses, polymerase chain reaction, cloning techniques, tissue culture procedures, and fluorescent antibody detection. This position will involve the generation and maintenance of cell lines, handling mice, and analyzing erythropoiesis in cell culture and animal models.
Responsibilities:
Cell culture
- Culture mouse and human erythroblasts, including monitoring of cultures using benzidine and trypan counts
- Generate and maintain cell lines for analyses using Crispr genome editing
- Genotype cell lines
- Analyze cultures using flow cytometric analyses
- Generate guide RNAs for Crispr experiments
- Design and perform cloning of constructs as directed
- Screen colonies generated via genome editing experiments using qPCR and western blot
- Assay mRNA and protein levels using qPCR and Western blot, respectively
- Organizing the work stations with the necessary supplies, including assembling any necessary plastic ware
- Cell staining and counting for flow cytometry experiments
- Performing routine laboratory analyses including (but not limited to) RT-PCR, western blot, immunofluorescence, cell culture, and microscopy.
- Routine cleaning of instrumentation and work area per protocol
- Restocking of work station supplies and ordering of supplies
- Assist with genotyping and colony maintenance
- Keeping current in the field through pertinent seminars and literature.
Qualifications:
- Associate's degree in appropriate discipline plus 2-3 years specialized experience in related field; or equivalent combination of education and experience required.
- BS/BA in biological/physical science or equivalent experience is preferred.
- A minimum of 1-year experience in laboratory science desired. Individuals with experience in handling mice and/or cytometry and cell culture are encouraged to apply required. Knowledge of University policy and procedures is desirable. Must have reliable/efficient transportation preferred.
How To Apply
All applicants must apply online.
EOE Minorities/Females/Protected Veterans/Disabled
Pay Range
Pay Range: $21.36 - $29.90 Hourly
The referenced pay range represents the minimum and maximum compensation for this job. Individual annual salaries/hourly rates will be set within the job's compensation range, and will be determined by considering factors including, but not limited to, market data, education, experience, qualifications, expertise of the individual, and internal equity considerations.
Created: 2024-09-27
Reference: 254645
Country: United States
State: New York
City: Rochester
ZIP: 14608
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