Laboratory Technician III - Pediatrics Neonatology

Classification Title:

Laboratory Technician III

Job Description:

This is a Lab Technician III position in a research lab developing lung biology. The Lab Technician III is expected to be able to:

1. Independently modify protocols to improve existing techniques or to incorporate new diagnostic procedures
2. Complete the execution of assigned experiments
3. Assist in maintaining a necessary rodent colony to achieve research objectives
4. Assist in performing rodent procedures as necessary to complete research objectives
5. Present researching findings to PI and at laboratory meetings
6. Maintain laboratory safety programs and train personnel in laboratory safety techniques
7. Maintain detailed records of experiments.

The Lab Technician III must be able to perform, mostly independently, the following essential functions:

Tissue culture:

Performing tissue culture/cell culture of primary cells or cell lines using aseptic techniques. Perform multiparameter flow cytometry and develop proficiency setting multicolor flow panels. Perform RNA and DNA isolation as well as in vitro transcription. Plasmid bacterial infection, plasmid transduction, and cloning are requirements for this position.

Animal Studies:

Participating in treating animals with pharmacological agents or immunotherapy through i.p., i.m., or i.v. injection. Plays a key role in regulatory management (IACUCs).

Molecular Experiments:

Including DNA & RNA extraction, protein assays, ELISA, western blot, PCR and

Histological Work/Analysis:

Collecting organs and other tissues and preparing for histopathology Following the preparation of slides, staining with standard histology stains such as H&E, willing to learn (if required) both immunohistochemistry and immunofluorescence technique. Conduct image capture with fluorescent microscope, confocal microscope, multiphoton confocal microscope and analysis of tissue following intervention studies.

Regulatory Compliance:

Ensures the laboratory is compliant with governing rules and regulations. Coordinate all IACUC and IRB (exempt) submissions/renewals. Ensure all EH&S standards are met. Train new laboratory members to ensure compliance standards are met.

Other Duties:

Maintaining and updating laboratory Standard Operating Procedures (SOPs). Any other duties, as needed, to fulfill the mission and to abide by the values of the College of Medicine. This will include performing tasks and experiments as directed by the Principal Investigator.

Expected Salary:

$23.90 - $26.00/hour

Minimum Requirements:

Associate's degree in an appropriate area and four years of related experience; or a high school diploma or equivalent and six years of related experience. Appropriate college course work or vocational/technical training may substitute at an equivalent rate for the required experience.

Preferred Qualifications:

• Demonstrated abilities in laboratory techniques required.
• Exposure to applicable computer techniques, including specific software application, is required.
• Appropriate communication skills required.

It will be given careful consideration to those applicants without the years of experience required above but who demonstrate strong research track record.

Special Instructions to Applicants:

In order to be considered, you must upload your cover letter and resume.

The University of Florida is committed to non-discrimination with respect to race, creed, color, religion, age, disability, sex, sexual orientation, gender identity and expression, marital status, national origin, political opinions or affiliations, genetic information and veteran status in all aspects of employment including recruitment, hiring, promotions, transfers, discipline, terminations, wage and salary administration, benefits, and training.

This is a time limited position.

Application must be submitted by 11:55 p.m. (ET) of the posting end date.

Health Assessment Required:
Yes

Advertised: 16 Aug 2024 Eastern Daylight Time
Applications close: 22 Aug 2024 Eastern Daylight Time